![](https://parts.igem.org/images/partbypart/icon_protein_domain.png)
Protein_Domain
Part:BBa_K4850006:Design
Designed by: Chai Chin Wei Group: iGEM23_NUS-Singapore (2023-10-10)
Rigid Protein Linker A(EAAAK)2A
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 7
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The increase in the linker length was found to reduce the fluorescence resonance energy, as stated in Arai et al. (2001).
Source
The sequence of this rigid protein linker was obtained from the Arai et al. (2001) paper.
References
[1] Arai, R., Ueda, H., Kitayama, A., Kamiya, N., & Nagamune, T. (2001). Design of the linkers which effectively separate domains of a bifunctional fusion protein. Protein Engineering Design & Selection, 14(8), 529–532. https://doi.org/10.1093/protein/14.8.529
[2] Chen, X., Zaro, J. L., & Shen, W. (2013). Fusion protein linkers: Property, design and functionality. Advanced Drug Delivery Reviews, 65(10), 1357–1369. https://doi.org/10.1016/j.addr.2012.09.039